4 0. Western blot analyses. A: Microplate Preparation. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. 40266 Email: drugdiscoverytech@lifetech. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The. 81). Figure 4: Transcreener ADP. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Readings were taken using a UV–Vis Spectramax M5 ® microplate reader (Molecular Device, USA). E Models. Category 2 and 3 microplate readers use gradient heating as well. 95. 2. 96-384. Figure 1. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. 00 USD. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. The Spectr. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . SDS-PAGE Assay. The SpectraMax M5e Multi. Molecular Devices. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The absorbance was recorded using a SpectraMax M5 microplate reader (Molecular Devices). 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax® Aquamax. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. RAW 264. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. A serial dilution of IgG standards was performed, using XP Media/L-glutamine as the diluent, to concentrations from 2. 0. Figure 1. 0 out of 5 $ 24,695. 1. l. RAW 264. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. edu) to be added as a trained user in iLab. Learn how to set up protocols, customize reports, export results, and more with this PDF document. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. 54 1. TR-FRET assay principle. Bookings are free, but you will need to use iLab to book. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Download PDF. Access this by going to Help >. ppr - M3, M4, M5, M5 SpectraMax readers (in SMP 4. AquaMax® 4000. The raw data from each individual experiment were normalized to vehicle-treated HK-2. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. The system. This application note describes how to. Parallel negative control assays had primary, secondary, or both antibodies omitted. Go to Product Details. STAKMAX. Thank you!. Wide range of applications. CAT activity was measured using SpectraMax M5/M5 Microplate Reader (Molecular Devices, MDS Analytical Technologies). The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. B: Selection of Excitation Wavelength. Label: provides information about the reader, such as line voltage rating, caution-ary information, serial number, etc. 04 to 3. 6-384 (96- and 384-well with pipetting) 6-384. Table 1. Fluorescence was measured (Ex 530/Em 590) in a SpectraMax M5 microplate reader. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. 63 1. Molecular Devices SpectraMax® series microplate readers. SpectraMax® M5e. Standard features include a. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The cells were then treated to induce apoptosis. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The bound bio-peptidomimetics were recognized by Eu 3+-SA (diluted using 1% BSA in TBS), and the fluorescence intensities were measured using a SpectraMax M5 microplate reader after adding 200 μL signal enhancement solution, which the parameters were set as the followed: excitation and emission wavelengths of 340 and 615 nm,. SpectraMax® Aquamax. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Self Service Available. Incubation. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. 303 unable to cal dark current. When wells are assigned to template groups, data will populate group tables where analysis canThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. KEY FEATURES • Five. Measurements of fluorescence and fluorescence polarization were performed on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA) with the excitation and emission wavelengths 544 nm and 590 nm, respectively. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Published Dec 16, 2020 | Updated Dec 16, 2020. Spectramax M5 Microplate Reader, supplied by Danaher Inc, used in various techniques. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Table of Contents. 4. 1. 05% Tween 20 using a Nunc ELISA plate washer. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Before you Begin Materials Required but Not Provided • Fresh, anhydrous. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] or greater. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. . 2. 87. SpectraMax® M5. • Tel. 3. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. 2. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. Choose from a three- (M3), four-. SpectraMax 250 User. We would like to show you a description here but the site won’t allow us. All reagents were brought to room temperature before use. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. SpectraMax® iD5. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. Bookings are free, but you will need to use iLab to book. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Description. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Plates were then shaken at room temperature for 10 min and the absorbance at 570/630 nm was measured by using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). Microplate Readers include the M3, M4, M5, and M5e models. SpectraMax® iD5. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. 67. This. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. The spectrophotometric. Step 2. Click SoftMax Pro Software User Guide. Supersedes 1. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. TR-FRET assay. SpectraMax Microplate Readers. Choose cables that meet the requirement described in this appendix. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 1. For the Live/Dead assay, they were treated. Add to Cart. Levels of immunoreactivity were normalized to. 1. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Validate the optical performance of your absorbance . We use Spectramax M5 microplate reader in our lab. Lift up the lid over the chamber to insert or remove a cuvette. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. The Multiskan Sky microplate reader is an absorbance plate reader. With optimized reagents, option to operate the. We would like to show you a description here but the site won’t allow us. 1 second. Page 28 Chapter 5: Read Modes and Read Types 1. ATP standard curves read on SpectraMax microplate readers. Flexible for 6-well to 384-well microplates and cuvettes. We would like to show you a description here but the site won’t allow us. TNF standard curve. A microplate reader asThe SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). The following document is intended to demonstrate setup of this instrument. Microplate Readers include the M3, M4,. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. View the Molecular Devices website. 00 USD. analysis, controls the SpectraMax Multi-Mode Microplate Readers. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 312 gain calibration failed. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. 1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 00 USD. When a FlexStation® 3 Multi-Mode Microplate Reader. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. 1 2. The SpectraMax L Microplate Reader was set up for endpoint reading with an inte-gration time of 1 second or 0. reader in the visible range between 405–750 nm with NIST and NMI. Read More. The dual monochromator optics allow. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Nontreated cells in DMEM medium were used as a control. 0 μL) instead of the enzyme solution was used as the sample blank group. FlexStation™ II. Spectramax Multi-Mode Microplate Reader Performance Specifications. The chamber contains springs that position the cuvette in the proper alignment for a read. 6. Step 1. 3 1. 49 2. 1. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. ) by nonlinear regression using. After 3 min of reaction, the fluorescence emission spectra of the mixed solutions were recorded using a multifunctional microplate reader (SpectraMax M5, Molecular Devices, USA) at the excitation wavelength (λ ex) of 485 nm. 6-384. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Maximum signal with lowest background. Please refer to the Obsolete Microplate Reader Models for more information. Fluorescence Microplate Readers. The assay applies fluorescence polarization to quantify Fc-containing IgG. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. Molecular Devices Spectramax M5 Microplate Reader. The dual monochromator optics allow the widest range of applications to be utilized for bior. A control experiment of the activity of the proposed enzyme/ZIF-8A particles without peptides against protease digestion was also performed. Turn on the microplate reader. Data are normalized to vehicle-treated. 00 USD. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. . Here the delta mP was 336, and Z’ factor was 0. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. SpectraMax iD5 Multi-Mode Microplate Reader. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Between steps, reactions were rinsed 3 times with TBS + 0. es •. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Table of Contents. Learn how you can utilize microplate readers to drive landmark discoveries. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The SpectraMax® iD3 Multi Mode Microplate . com. SpectraMax® ABS Plus. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplateinstalled if you are using a SpectraMax ABS microplate reader **SoftMax Pro Software version 7. and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Uncontrolled Copy for Training and Reference Purposes Only . Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 5 Dual-luciferase reporter assay. Small, unbound molecules rotate rapidly in. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. After being incubated in the dark at 60 ℃ overnight, the supernatants were centrifuged and optical densities were measured at 750, 665, 652 and 470 nm using the SpectraMax M5 microplate reader (Molecular Devices,. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!The microplate readers in SpectraMax M5 series can also provide high-throughput capabilities with its automated microplate handling and storage systems. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 4. 4. 0. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. analysis, controls the SpectraMax Multi-Mode Microplate Readers. Add to Cart. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. free tracer decreases, resulting in. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). Page 1 of 8 . SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. 7°C and the bottom to 36. 1 Version 7. Fill a clean plate with water. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Cuvette port functionality can also be controlled using SoftMax Pro Software. The SpectraMax M2 and M2e are versatile and reliable. Microplate Capacity. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. 5–100mg/L (ValitaTiter) or 100–2000mg/L (ValitaTiter Plus). 40266 Email: drugdiscoverytech@lifetech. Then, the fluorescence change rates (F 0 − F)/F 0 were calculated, and the selectivity histogram. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. FlexStation™ I. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. Key applications: ELISA, nucleic acid, endotoxin testing. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. It measures the optical density (OD) of samples in a microplate or in a cuvette at aThe cells were then washed three times with PBS, and the fluorescent intensity of the cells was measured with a SpectraMax M5 microplate reader (Molecular Devices, USA) at an excitation wavelength of 488 nm and emission wavelength of 525 nm. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. Wide range of applications The VersaMax reader covers. The absorbance was measured at 490 nm using a SpectraMax® M5 microplate reader (Molecular Devices, USA). SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Supplier: Molecular Devices. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. , Sunny-vale, CA). With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Equal amount of proteins were loaded and resolved using denaturing gel and analyzed by western blot using antibodies recognizing Akt, rS6, mOTR and. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Microplate Readers include the M3, M4, M5, and M5e models. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. com. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The dual monochromator optics allow the widest range of applications to be. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. 5 12. Qualify the luminescence performance of the SpectraMax . TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The integration time suggested is 5-10 sec. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. The following document is intended to demonstrate setup of this instrument. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 6: bioNova científica, s. ,. The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. However, when a specific probe is not available, or it causes off-target effects on the. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 0 out of 5 $ 2,499 $ 1,999. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. 1 is GxP edition only; Protocols for the ABS1 Validation Plate. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. PMT sensitivity was set to AutoRange with a target calibration wavelength of 470 nm, and the plate was dark adapted for 1 minute prior to reading. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. Figure 1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.